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Dietary lutein supplementation on diet digestibility and blood parameters of dogs Ciência Rural
Alarça,Laís Guimarães; Murakami,Fabiane Yukiko; Félix,Ananda Portella; Krabbe,Everton Luis; Oliveira,Simone Gisele de; Silva,Sebastião Aparecido Borges da.
ABSTRACT: The objective of the present study was to evaluate the digestibility and the immunomodulatory effects of diets supplemented with lutein in dogs. Sixteen adult dogs were distributed in two groups (control and test) in a completely randomized design. Dogs were fed for 120 days with iso-nutritive diets, which only difference was the inclusion of 45g lutein/kg in the diet fed to the test group. Blood samples were collected in the beginning and at the end of the experimental period to evaluate total lymphocyte, lymphocyte proliferation index, and CD4+ and CD8+ lymphocyte counts. In order to evaluate apparent diet digestibility, dogs were housed in metabolic cages for ten days (five days of adaptation and five for faeces total collection). Coefficients...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carotenoids; CD4+; CD8+; Immunity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202195
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Stability of the pigmentation of egg yolks enriched with omega-3 and carophyll stored at room temperature and under refrigeration R. Bras. Zootec.
Barbosa,Vanessa Camarinha; Gaspar,Arlene; Calixto,Ligia Fátima Lima; Agostinho,Tarcísio Simões Pereira.
The objective of this experiment was to study yolk pigmentation stability in omega 3 (ω-3) enriched eggs stored under different temperatures in zeaxanthin equivalent (by using visible light spectrophotometer) and visual score (with values obtained by yolk color fan) and to verify the correlation among those methodologies. Three hundred big eggs from Isa Brown hens were distributed into two lots each one containing 150 eggs, stored at room temperature (26.5ºC) or under refrigeration (7.9ºC) for 35 days. It was used a completely randomized design in a 2 × 6 factorial arrangement (storage temperature × storage period). Pigmentation observed with the yolk color fan was steady during the experiment period when eggs were kept under refrigeration and it reduced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carotenoids; Colour fan; Spectrophotometer; Yolk.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000700020
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Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production ArchiMer
Ben Amor, Faten; Elleuch, Fatma; Ben Hlima, Hajer; Garnier, Matthieu; Saint-jean, Bruno; Barkallah, Mohamed; Pichon, Chantal; Abdelkafi, Slim; Fendri, Imen.
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel...
Tipo: Text Palavras-chave: Proteome; Dunaliella; 18S rRNA gene; Carotenoids; Bidimensional gel electrophoresis; Mass spectrometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00402/51398/51973.pdf
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Post-harvest physicochemical profile and bioactive compounds of 19 bananas and plantains genotypes Bragantia
Borges,Cristine Vanz; Amorim,Edson Perito; Leonel,Magali; Gomez,Hector Alonzo Gomez; Santos,Thais Paes Rodrigues dos; Ledo,Carlos Alberto da Silva; Belin,Matheus Antônio Filiol; Almeida,Samara Lopes de; Minatel,Igor Otavio; Lima,Giuseppina Pace Pereira.
ABSTRACT Nineteen genotypes of bananas and plantains were analysed in order to differentiate the subgroups and/or groups of consumption or industrial use. Genotypes of banana and plantain from different genomic groups and in three ripening stages (2, 5 and 7) were studied in relation to physical and physicochemical characteristics, including bioactive compounds. Furthermore, with the obtained data analysed by multivariate statistical analyses (Principal Component Analysis) it was possible to relate all analysed characteristic profile of samples with the different genotype. The three ripening stages were differentiate by total soluble solids, titratable acidity, chrome (C*) and the carotenoids contents. ‘Ney Poovan’ contain high total soluble solid content...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Musa spp; Carotenoids; Vitamin C; Cooking banana.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000200284
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Non-destructive analysis of photosynthetic pigments in cotton plants - doi: 10.4025/actasciagron.v33i4.10926 Agronomy
Brito, Giovani Greigh; Empresa Brasileira de Pesquisa Agropecuária; Sofiatti, Valdinei; Empresa Brasileira de Pesquisa Agropecuária; Brandão, Ziany Neiva; Empresa Brasileira de Pesquisa Agropecuária; Silva, Vivianny Belo; Empresa Brasileira de Pesquisa Agropecuária; Silva, Franklin Magnum; Empresa Brasileira de Pesquisa Agropecuária; Silva, Dalva Almeida; Empresa Brasileira de Pesquisa Agropecuária.
Analytical techniques used to extract chlorophyll from plant leaves are destructive and based on the use of organic solvents. This study proposes a non-destructive quantification of the photosynthetic pigment concentration in cotton leaves using two portable chlorophyll meters, the SPAD-502 and the CLOROFILOG 1030. After obtaining 200 leaf discs, each with an area of 113 mm2, the greening rate in each disc was determined by the average of five readings from both meters. Immediately after measurement, 5 mL of dimethyl sulfoxide (DMSO) was added, and the samples were kept in a water bath at 70ºC for 30 min. After cooling, 3 mL of the liquid extract was used for analyses by spectrophotometry at 470, 646 and 663 nm. Mathematical models were adjusted from...
Tipo: Pesquisa Empirica de Campo Palavras-chave: Fisiologia de plantas cultivadas Gossypium hirsutum; Chlorophyll; Carotenoids; Cotton 5.01.03.06-7 Gossypium hirsutum; Chlorophyll; Carotenoids; Cotton.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10926
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Processamento de polpas e sucos de frutas por processos com membranas. Infoteca-e
CABRAL, L. M. C.; CANDÉA, I. V.; CANDÉA, T. V.; MECENAS, A. S.; PINTO, T. de M.; MATTA, V. M. da.
bitstream/item/74990/1/pub-204.pdf
Tipo: Documentos (INFOTECA-E) Palavras-chave: Polpa de cajá; Suco de amora; Microfiltração; Suco de melão; Osmose inversa; Tecnologia de membrana; Clarification.; Carotenóide; Processamento; Spondias Mombin.; Microfiltration; Carotenoids; Fruit juices; Melons; Blackberries; Processing technology..
Ano: 2010 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/874618
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Pigmentos carotenoides del camarón Artemesia longinaris Bate (Crustacea, Decapoda, Penaeidae) OceanDocs
Carreto, J.I.; Carignan, M.O..
Caronetoids from caparace and internal organs of Artemesia longinaris were isolated and identified. The following carotenoid pigments were found in caparace: luteine, tunaxanthin diester, astaxanthin monoester and free astaxanthin. A much greater variety of pigments was isolated from internal organs: Ó- doradexanthin, a fucoxanthin derivate, chlorophylls degradation products and various xanthophylls in trace amounts. These pigments are of dietary origin. In the natural environment, A.longinaris takes preformed astaxanthin from the diet and transfers it to body tissues.
Tipo: Journal Contribution Palavras-chave: Pigments; Carotenoids; Absorption spectra; Pigments; Carotenoids; Http://aims.fao.org/aos/agrovoc/c_5875; Http://aims.fao.org/aos/agrovoc/c_1333.
Ano: 1984 URL: http://hdl.handle.net/1834/2048
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QUALITY OF CAGAITA POWDER BY FOAM LAYER DRYING METHOD AND DIFFERENT FOAMER AGENTS REA
Cavalcante,Maisa D.; Belisário,Celso M.; Oliveira,Daniel Emanuel C. de; Maia,Geisa Priscilla A. G..
ABSTRACT One of the factors that hinder the usability of Cerrado fruits is their perishable nature, requiring industrial processing to ensure prolonged shelf life. This study aims to determine the optimal foaming agent for industrial processing of this fruit, and to evaluate the physicochemical characteristics and antioxidant activity of dried cagaita powder (Eugenia dysenterica DC) in foam layers at temperatures of 40, 50, 60, and 70 °C. The effectiveness of three foaming agents at various concentrations were tested, and suitable agents were identified based on a factorial analysis of density, expansion, and stability. The physicochemical characteristics of the powder, along with its vitamin A and C and total carotenoid content and antioxidant activity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado fruits; Cagaita powder; Bioactive compounds; Antioxidant activity; Vitamin C; Vitamin A; Carotenoids; Foam layer drying.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162020000300381
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Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation Braz. J. Plant Physiol.
Chakraborty,Usha; Pradhan,Bhumika.
Five varieties of wheat (Triticum aestivum L.) - KW, UP 2752, PBW 343, SO and LV - were subjected to water stress and sampling was done on the 3rd, 6th and 9th day of stress. RWC decline in KW, UP 2752 and PBW 343 (36.65, 42.34 and 40.75% respectively) was comparatively lesser than in LV and SO (52.93 and 52.67% respectively). In all varieties tested, three antioxidant enzymes (POX, APOX and GR) showed an initial increase. The activity of POX and GR increased with the increase in the duration of stress in KW, UP 2752 and PBW 343, while the activity of APOX declined. However, CAT and SOD showed an initial increase in these varieties, whereas it declined in SO and LV with increase in the period of stress. Accumulation of H2O2 declined during prolonged water...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Carotenoids; Drought; Lipid peroxidation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200005
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Production and quality of carrot in function of split application of nitrogen doses in top dressing Horticultura Brasileira
Colombari,Lidiane F; Lanna,Natália BL; Guimarães,Leysimar RP; Cardoso,Antonio Ismael Inácio.
ABSTRACT Fertilizers supply in the appropriate period and dose is important to obtain satisfactory production. In this research we studied the effect of different split applications and doses of nitrogen in top dressing on the production of the carrot and the physicochemical characteristics of the roots. Nine treatments, resulting from a factorial of 4x2+1 (control without nitrogen fertilization), were evaluated in the randomized blocks experimental design, with four replications. The first factor were four nitrogen (N) doses in top dressing (60, 90, 120 and 150 kg ha-1) and the second were two forms of splitting [three equal applications (1/3+1/3+1/3 of the dose) and one with increasing proportions (1/6+2/6+3/6 of the dose) applied 15, 30 and 50 days...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Daucus carota; Carotenoids; Fertilization; Root quality; Yield.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300306
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Physiological characteristics of the Atlantic Forest native bromeliads: Nidularium campo-alegrense Leme and Aechmea ornata Baker - doi: 10.4025/actascibiolsci.v36i1.20215 Biological Sciences
Corredor Prado, Jenny Paola; Universidade Federal de Santa Catarina; Morales Londonõ, Diana Marcela; Universidade Federal de Santa Catarina; Almeida, Vivian; Universidade Federal de Santa Catarina; Brunetto, Gustavo; Universidade Federal de Santa Catarina; Pescador, Rosete; Universidade Federal de Santa Catarina.
Despite the ecological importance of bromeliads, the basic knowledge about the physiological aspects in some species is poorly understood. The aim of this study was to physiologically characterize the species Nidularium campo-alegrense Leme and Aechmea ornata Baker to contribute to a better understanding of their metabolic processes. From mature leaves of bromeliads N. campo-alegrense and A. ornata held at the Agricultural Sciences Center of the Federal University of Santa Catarina, it was quantified photosynthetic pigments, carbohydrates and starch, and determined stomata and trichome density. The chlorophyll and carotenoids content did not differ significantly between species. However, the total carbohydrates content of A. ornata (3.2 mg g-1 FM) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.03.00-9 Bromeliaceae; Carbohydrates; Carotenoids; Chlorophyll Fisiologia vegetal.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/20215
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Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies Electron. J. Biotechnol.
Dayananda,Chandrappa; Sarada,Ravi; Kumar,Vinod; Ravishankar,Gokare Aswathanarayana.
Samples of green colonial unicellular microalga Botryococcus braunii were collected from Bear Shola Falls at Kodaikanal (latitude 10.31 N and longitude 77.32 E), Tamil Nadu, India. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and DNA structural resemblance with the known strain to confirm its identity. Inter simple sequence repeats (ISSR) finger printing revealed strong genetic similarity among the authentic strain (B. braunii N-836) and the Indian isolated strain (B. braunii CFTRI- Bb1) from Kodaikanal. The type of hydrocarbons produced by the Kodaikanal isolates were analyzed and identified as saturated hydrocarbons in the range of C21 to C33 by GCMS. Tetracosane and octacosane were found as the major...
Tipo: Journal article Palavras-chave: Biomass; Carbon dioxide; Carotenoids; Fatty acids; Inter simple sequence repeats; Saturated hydrocarbons.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100008
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Effect of industrial processing for obtaining guava paste on the antioxidant compounds of guava (Psidium guajava l.) ‘Paluma’ cv. Rev. Bras. Frutic.
Del’Arco,Ana Paula Wolf Tasca; Sylos,Célia Maria de.
Abstract Some foods constituents are subject to change during thermal and/or industrial processing. In the fruit processing industry, guava pulp is used during guava off season to obtain guava paste, produced with the addition of sugar and citric acid, subjected to various thermal processes. This study evaluated the effect of industrial processing to obtain guava paste on the content of ascorbic acid, total carotenoids, total phenolic and total flavonoid present in guava ‘Paluma’ cv. The results showed that there was an increase of antioxidants in guava pulp (15% ascorbic acid, 59% total carotenoids, 8% total phenolics and 54.5% total flavonoids), probably due to their increased concentration as a result of water loss during processing. There was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascorbic Acid; Carotenoids; Phenolics; Antioxidant Activity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000200901
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Inclusion of canthaxanthin and 25-hydroxycholecalciferol in the diet of broiler breeders on performance and incubation parameters Ciência Rural
Duarte,Vinicius; Minafra,Cibele Silva; Santos,Fabiana Ramos dos; Perim,Francisco dos Santos.
The objective of this experiment was to evaluate the effects of a diet containing canthaxanthin and 25-hydroxycholecalciferol (25-OH-D3) on the production and reproductive performances of Cobb 500 broiler breeders aged 53 to 61 weeks. The study included 36,000 Cobb 500 female and 3,600 male broiler breeders aging 51 weeks. The birds were housed in three poultry houses divided into four plots, totaling 12 experimental units each containing 3,000 females and 300 males. The birds received the following treatments: control diet (without the studied additives) or the control diet with the addition of 60mg kg-1feed of 25-(OH)-D3 and canthaxanthin feed (6mg of canthaxanthin and 2,760,000IU of 25-(OH)-D3 per kg of feed) per the supplier's recommendations. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carotenoids; Vitamin D; Broiler breeders; Hatching; Egg production.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001102050
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BIOACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF PINEAPPLE FRUIT OF DIFFERENT CULTIVARS Rev. Bras. Frutic.
FERREIRA,ESTER ALICE; SIQUEIRA,HELOISA ELIAS; BOAS,EDUARDO VALERIO VILAS; HERMES,VANESSA STAHL; RIOS,ALESSANDRO DE OLIVEIRA.
ABSTRACT Pineapple is widely consumed and appreciated not only due to its taste and aroma, and also to its nutritional, functional and antioxidant properties, including its vitamin C and carotenoid contents. Brazil is one of the largest world’ pineapple producer, and Pérola and Smooth Cayenne cultivars are the most commonly grown and marketed, but their susceptibility to fusariosis can compromise cultivation. New cultivars resistant to this pathogen are available to meet the demands of pineapple producers and consumers. The aim of this study was to evaluate the content of bioactive compounds and antioxidant activity of pineapple fruits of Imperial, Victoria, IAC Fantástico and Gomo de Mel cultivars, as well as traditional Pérola and Smooth Cayenne...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosuse; Fusariosis; Vitamin C; Phenolics; Carotenoids.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000300801
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BIOACTIVE COMPOUNDS DURING THE MATURATION OF FOUR FRUITS NATIVE TO THE RESTINGA FOREST OF CEARA Rev. Bras. Frutic.
GONÇALVES,NIGÉRIA PEREIRA; LUCENA,ELISEU MARLÔNIO PEREIRA DE; BONILLA,ORIEL HERRERA; SILVEIRA,MÁRCIA RÉGIA SOUZA DA; TAVARES,FRANCISCA JÉSSICA COSTA.
ABSTRACT Fruits provide not only essential nutrients for food, but also bioactive compounds that promote health benefits and help reducing the risk of developing non-communicable chronic diseases. In this sense, this work aimed at quantifying bioactive compounds during the maturation of four fruits native to the Restinga forest of Ceara. Myrtle fruits (Eugenia punicifolia (Kunth) DC.) were collected at the Botanical State Park of Ceara, Caucaia-CE, and guajiru (Chrisobalanus icaco L.), manipuça (Mouriri cearensis Huber) and murici-pitanga fruits (Byrsonima gardneriana A. Juss.) at the Botanical Garden of São Gonçalo, São Gonçalo do Amarante-CE. Fruits were collected at different stages (E) and transported to the Laboratory of Plant Ecophysiology, being...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascorbic acid; Chlorophyll; Carotenoids; Anthocyanins; Yellow flavonoids.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000500303
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Effect of Bentazon on Growth and Physiological Responses of Marine Diatom: Chaetoceros gracilis ArchiMer
Hourmant, Annick; Amara, Anis; Pouline, Patrick; Durand, Gael; Arzul, Genevieve; Quiniou, Francoise.
The herbicide bentazon (CASRN 25057-89-0) is extensively used in agriculture in Brittany (France) to replace atrazine. Bentazon is not readily adsorbed by soil and therefore it enters adjacent freshwater ecosystems, making its way to estuarine and marine waters areas. Information regarding its effects on marine ecosystems is scarce. Phytotoxicity assessments were conducted in the laboratory on the common diatom Chaetoceros gracilis using both the active ingredient and its formulated product (Basamais). The 3 day EC50 Using cell counts were, respectively, 150 mg/L and 60 mu g/L for bentazon active ingredient and for bentazon-formulated while cellular volume was increased. Although bentazon is known as a photosystem II inhibitor, it produced an increase of...
Tipo: Text Palavras-chave: Photosynthesis; Marine diatom; Growth; Chlorophylls; Chaetoceros gracilis; Carotenoids; Bentazon; ATP.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6637.pdf
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Role of pH on antioxidants production by Spirulina (Arthrospira) platensis BJM
Ismaiel,Mostafa Mahmoud Sami; El-Ayouty,Yassin Mahmoud; Piercey-Normore,Michele.
Abstract Algae can tolerate a broad range of growing conditions but extreme conditions may lead to the generation of highly dangerous reactive oxygen species (ROS), which may cause the deterioration of cell metabolism and damage cellular components. The antioxidants produced by algae alleviate the harmful effects of ROS. While the enhancement of antioxidant production in blue green algae under stress has been reported, the antioxidant response to changes in pH levels requires further investigation. This study presents the effect of pH changes on the antioxidant activity and productivity of the blue green alga Spirulina (Arthrospira) platensis. The algal dry weight (DW) was greatly enhanced at pH 9.0. The highest content of chlorophyll a and carotenoids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Carotenoids; Phycocyanin; Total phenolic content; Radical scavenging activity; Antioxidant enzymes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200298
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Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage Ciência Rural
Junqueira,Mateus da Silva; Simões,Adriano do Nascimento; Sediyama,Tocio; Côrrea,Paulo Cesar; Puschmann,Rolf.
The aim of this study was to evaluate the effects of fresh-cut in stick form of two cassava cultivars on their biochemical and antioxidant characteristics, at two harvest times. The fresh cut cassava sticks were packaged in polypropylene, maintained at 5±1ºC, with 90±5% relative humidity for 12 days. The concentration of carotenoids, total soluble phenolic compounds and the activity of phenylalanine ammonia lyase and antioxidant capacity were significantly higher for the sticks of cultivar 'Amarela' cassava than for the sticks of cultivar 'Cacau'. The concentrations of carotenoids, total soluble phenolic compounds, and the activity of phenylalanine ammonia lyase and antioxidant capacity were all significantly greater when harvested at 14-month of age....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta Crantz; Carotenoids; PAL; Quality; Antioxidant capacity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000701284
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Microencapsulation of pequi pulp oil by complex coacervation Rev. Bras. Frutic.
Justi,Priscilla Narciso; Sanjinez-Argandoña,Eliana Janet; Macedo,Maria Lígia Rodrigues.
Abstract Pequi pulp oil, Caryocar brasiliense, is rich in carotenoids, antioxidant compound easily oxidized by the presence of heat, light and oxygen. In order to improve its stability, pequi oil was microencapsulated by complex coacervation using gelatin and Arabic gum as encapsulating agents. Twenty formulations were prepared using a 23 central composite rotational design. The influence of temperature, stirring velocity and core material in the oil coacervation were evaluated, aiming to preserve carotenoids present in the oil. The best yield values and carotenoids content were obtained at the midpoint of the design (7.5g core, 15.000rpm and 50°C). Particles showed asymmetric distribution, with diameter ranging from 15 to 145 µm and the efficiency of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caryocar brasiliense; Carotenoids; Retention.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000200904
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